The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR items were digested and cloned right into a pET21a+ vector applying NdeI and XhoI restriction enzymes. A degree mutation was launched by the quick change https://manuelgzslc.educationalimpactblog.com/48759575/xeruborbactam-for-dummies